Difference between revisions of "Part:BBa K4960003"
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<partinfo>BBa_K4960003</partinfo><br>
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<partinfo>BBa_K4960003 short</partinfo><br>
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Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices and present in both bacteria and archaea.[1]In structure, PVCs are macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2]Pvc3 consists of the sheath with Pvc2 and Pvc4.
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Pvc3 is a part of the sheath.
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<!-- Add more about the biology of this part here-->
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===Usage and Biology===
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Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices and present in both bacteria and archaea.[1]In structure, PVCs are macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2]
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Pvc3 consists of the sheath with Pvc2 and Pvc4.
 
Pvc3 contains an extra protrusion domain, which is used for tailfiber docking, is in a comparable position of domain III in the T6SS sheath.
 
Pvc3 contains an extra protrusion domain, which is used for tailfiber docking, is in a comparable position of domain III in the T6SS sheath.
 
A part of sheath, comprises alternating Pvc2 and Pvc3 subunits, which both interact with Pvc1. At the end of assembly, Pvc3 starts to assemble along the inner tube for the maturation of PVC particles with Pvc2.
 
A part of sheath, comprises alternating Pvc2 and Pvc3 subunits, which both interact with Pvc1. At the end of assembly, Pvc3 starts to assemble along the inner tube for the maturation of PVC particles with Pvc2.
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===Usage and Biology===
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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===Functional Parameters===
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===Experimental Validation===
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This part is validated through functional testing.
 
<partinfo>BBa_K4960003 parameters</partinfo>
 
<partinfo>BBa_K4960003 parameters</partinfo>
 
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===Reference===
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[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7. Epub 2023 Mar 29. PMID: 36991127; PMCID: PMC10097599.
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[2]Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell. 2019 Apr 4;177(2):370-383.e15. doi: 10.1016/j.cell.2019.02.020. Epub 2019 Mar 21. PMID: 30905475.

Revision as of 18:19, 10 October 2023


pvc3
Pvc3 is a part of the sheath.

Usage and Biology

Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices and present in both bacteria and archaea.[1]In structure, PVCs are macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2] Pvc3 consists of the sheath with Pvc2 and Pvc4. Pvc3 contains an extra protrusion domain, which is used for tailfiber docking, is in a comparable position of domain III in the T6SS sheath. A part of sheath, comprises alternating Pvc2 and Pvc3 subunits, which both interact with Pvc1. At the end of assembly, Pvc3 starts to assemble along the inner tube for the maturation of PVC particles with Pvc2. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 452
    Illegal XhoI site found at 736
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7. Epub 2023 Mar 29. PMID: 36991127; PMCID: PMC10097599. [2]Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell. 2019 Apr 4;177(2):370-383.e15. doi: 10.1016/j.cell.2019.02.020. Epub 2019 Mar 21. PMID: 30905475.