Difference between revisions of "Part:BBa K200019"
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This is the testing construct used by Imperial College's 2009 iGEM team to assess the two inducible promoters pLacI and pCstA through the expression of reporter molecules RFP and GFP in [http://2009.igem.org/Team:Imperial_College_London <i>The E.ncapsulator</i>] project.  
 
This is the testing construct used by Imperial College's 2009 iGEM team to assess the two inducible promoters pLacI and pCstA through the expression of reporter molecules RFP and GFP in [http://2009.igem.org/Team:Imperial_College_London <i>The E.ncapsulator</i>] project.  
  
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The subparts used to make this composite are [https://parts.igem.org/Part:BBa_J5526 BBa_J5526] and [https://parts.igem.org/Part:BBa_K00018 BBa_K00018].
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The subparts used to make this composite are [https://parts.igem.org/Part:BBa_J5526 BBa_J5526] and [https://parts.igem.org/Part:BBa_K200018 BBa_K200018].
  
 
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Revision as of 21:49, 19 October 2009

pLacI+RBS+RFP+TT+pCstA+RBS+GFP+TT

This is the testing construct used by Imperial College's 2009 iGEM team to assess the two inducible promoters pLacI and pCstA through the expression of reporter molecules RFP and GFP in [http://2009.igem.org/Team:Imperial_College_London The E.ncapsulator] project.

The subparts used to make this composite are BBa_J5526 and BBa_K200018.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 636
    Illegal AgeI site found at 748
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1733