Difference between revisions of "Part:BBa K4729707"
Line 4: | Line 4: | ||
== Introduction == | == Introduction == | ||
+ | |||
+ | == The molecular machinery of plant transformation == | ||
+ | |||
+ | == The problem == | ||
+ | |||
+ | == The virG Master-Switch construct == | ||
+ | |||
+ | === Constitutive expression === | ||
+ | |||
+ | === Inducible expression === | ||
+ | |||
+ | === Sodium Salicylate inhibits cell growth === | ||
+ | |||
+ | === Cross-talk assay === | ||
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Revision as of 16:50, 10 October 2023
Contents
Ptac + VirG TiBo542 N80D + repABCa
Introduction
The molecular machinery of plant transformation
The problem
The virG Master-Switch construct
Constitutive expression
Inducible expression
Sodium Salicylate inhibits cell growth
Cross-talk assay
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 3840
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 4468
Illegal PstI site found at 3840 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1741
Illegal BamHI site found at 262
Illegal BamHI site found at 1917
Illegal BamHI site found at 2209
Illegal XhoI site found at 227
Illegal XhoI site found at 3480
Illegal XhoI site found at 3834
Illegal XhoI site found at 7176 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 3840
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 3840
Illegal NgoMIV site found at 111
Illegal NgoMIV site found at 2054
Illegal NgoMIV site found at 3894
Illegal NgoMIV site found at 4119
Illegal NgoMIV site found at 4224
Illegal NgoMIV site found at 5254
Illegal AgeI site found at 5733 - 1000COMPATIBLE WITH RFC[1000]