Difference between revisions of "Part:BBa K175005:Experience"
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| − | | Set # || Conjugation Efficiency for | + | | Set # || Conjugation Efficiency for K175005 on pSB4C5 || Conjugation Efficiency for wild R751 |
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| 1 || 0.00177 || 0.0141 | | 1 || 0.00177 || 0.0141 | ||
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The reason for the lower efficiency of K175005 on pSB4C5 compared to R751 is the presence of the entry exclusion protein trbK. If a receiver cell gets K175005 on pSB4C5, it can still receive R751 afterward. But if a receiver cell gets R751, it will not accept K175005 on pSB4C5 afterward because trbK expression will block incoming transfers. | The reason for the lower efficiency of K175005 on pSB4C5 compared to R751 is the presence of the entry exclusion protein trbK. If a receiver cell gets K175005 on pSB4C5, it can still receive R751 afterward. But if a receiver cell gets R751, it will not accept K175005 on pSB4C5 afterward because trbK expression will block incoming transfers. | ||
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===User Reviews=== | ===User Reviews=== | ||
Revision as of 21:31, 19 October 2009
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K175005
Characterization of BBa_K175005
A conjugation test was done using the TUDelft iGEM 2009 [http://2009.igem.org/Team:TUDelft/ConjugationProtocol conjugation protocol] to verify that K175005 on a pSB4C5 low-copy backbone was transmitted during conjugation.
Donors: [http://www.cbs.knaw.nl/databases/nccb/search_bac_plas.aspx NCCBNr# 2350] cells with R751 and K175005 on pSB4C5 (TRI+CAM)
Receivers: DH5 α cells with J23100 (AMP)
Transconjugants A: DH5 α cells with R751 and J23100 (TRI+AMP)
Transconjugants B: DH5 α cells with K175005 on pSB4C5 and J23100 (CAM+AMP)
The following table shows the calculated conjugation efficiencies (raw data [http://2009.igem.org/Team:TUDelft/19_August_2009#Calin here]):
| Set # | Conjugation Efficiency for K175005 on pSB4C5 | Conjugation Efficiency for wild R751 |
| 1 | 0.00177 | 0.0141 |
| 2 | 0.00224 | 0.0476 |
| 3 | n/a | 0.0356 |
The reason for the lower efficiency of K175005 on pSB4C5 compared to R751 is the presence of the entry exclusion protein trbK. If a receiver cell gets K175005 on pSB4C5, it can still receive R751 afterward. But if a receiver cell gets R751, it will not accept K175005 on pSB4C5 afterward because trbK expression will block incoming transfers.
User Reviews
UNIQea6701d958abde91-partinfo-00000000-QINU UNIQea6701d958abde91-partinfo-00000001-QINU