Difference between revisions of "Part:BBa K4759224:Design"
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Revision as of 16:34, 10 October 2023
T7-RBS1-petH-RBS2-petF(D21F)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1336
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1336
Illegal NotI site found at 1109 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1336
Illegal BglII site found at 1647
Illegal BamHI site found at 1330 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1336
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1336
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Site-directed mutagenesis of the PetF protein