Difference between revisions of "Part:BBa K4759223:Design"

 
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===Design Notes===
 
===Design Notes===
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1
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Site-directed mutagenesis of the PetF protein
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===Source===
 
===Source===
  
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1
 
  
 
===References===
 
===References===

Revision as of 16:31, 10 October 2023


T7-RBS1-petH-RBS2-petF(D21Y)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1336
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1336
    Illegal NotI site found at 1109
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1336
    Illegal BglII site found at 1647
    Illegal BamHI site found at 1330
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1336
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1336
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Site-directed mutagenesis of the PetF protein


Source

References