Difference between revisions of "Part:BBa K4593007:Design"

 
 
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__NOTOC__
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<partinfo>BBa_K4593007 short</partinfo>
  
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<partinfo>BBa_K4593007 SequenceAndFeatures</partinfo>
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===Design Notes===
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We added biotin at the 3’ end to test the affinity of the aptamer
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===Source===
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In 2016, Regina Stoltenburg and her team discovered PA#2/8 is an aptamer binding the S.aureus protein A. We employed a computational approach using the trained BERT language model in a dry lab setting. Through this screening of large amount of aptamer mutants, we discovered this aptamer as effective.
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===References===
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[1] Stoltenburg, R., Krafčiková, P., Víglaský, V., & Strehlitz, B. (2016). G-quadruplex aptamer targeting Protein A and its capability to detect Staphylococcus aureus demonstrated by ELONA. Scientific Reports, 6(1). https://doi.org/10.1038/srep33812

Latest revision as of 16:28, 10 October 2023

PA#2/8-B


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We added biotin at the 3’ end to test the affinity of the aptamer


Source

In 2016, Regina Stoltenburg and her team discovered PA#2/8 is an aptamer binding the S.aureus protein A. We employed a computational approach using the trained BERT language model in a dry lab setting. Through this screening of large amount of aptamer mutants, we discovered this aptamer as effective.

References

[1] Stoltenburg, R., Krafčiková, P., Víglaský, V., & Strehlitz, B. (2016). G-quadruplex aptamer targeting Protein A and its capability to detect Staphylococcus aureus demonstrated by ELONA. Scientific Reports, 6(1). https://doi.org/10.1038/srep33812