Difference between revisions of "Part:BBa K4830002:Design"

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===Design Notes===
 
===Design Notes===
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The provided sequence contains the following parts: U6 promoter, spacer, guide RNA scaffold, rttpbs (A to C), polyA tail.
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The design of RNF2 pegRNA includes the U6 promoter, spacer, guide RNA scaffold, rttpbs and polyA tail.
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===Source===
 
===Source===

Revision as of 15:22, 10 October 2023


RNF2 AtoC pegRNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The design of RNF2 pegRNA includes the U6 promoter, spacer, guide RNA scaffold, rttpbs and polyA tail.

Source

The original RNF2 AtoC pegRNA sequence containing spacer and rttpbs was reported in Prime Editor 2 paper cited in References. We used those sequences and with the help of and TWIST Bioscience synthesized the parts, which were further pieced together with 65777 backbone in our lab.

References

Anzalone AV, Randolph PB, Davis JR, Sousa AA, Koblan LW, Levy JM, et al. Search-and-replace genome editing without double-strand breaks or donor DNA. Nature. 2019 Oct 21;576.