Difference between revisions of "Part:BBa K200004"
 
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<partinfo>BBa_K200004 short</partinfo>
 
<partinfo>BBa_K200004 short</partinfo>
  
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This sequence codes for the restriction enzyme TaqI.
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This sequence codes for the restriction enzyme TaqI. It will be used in the composite part [https://parts.igem.org/wiki/index.php/Part:BBa_K200010 BBa_K200010] by Imperial iGEM 2009 in <i>[http://2009.igem.org/Team:Imperial_College_London The E.ncapsulator]</i> project.
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This is a Type II restriction enzyme that recognises the sequence TCGA.  Its activity can be blocked by dam methylation. <br>
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The working temperature is 65°C. <br>
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As part of the Imperial 2009 iGEM E.ncapsulator project, TaqI was used along with DpnII to cut up the genetic material within the cell, completing the cell death module.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 21:18, 19 October 2009

TaqI

This sequence codes for the restriction enzyme TaqI. It will be used in the composite part BBa_K200010 by Imperial iGEM 2009 in [http://2009.igem.org/Team:Imperial_College_London The E.ncapsulator] project.

This is a Type II restriction enzyme that recognises the sequence TCGA. Its activity can be blocked by dam methylation.

The working temperature is 65°C.

As part of the Imperial 2009 iGEM E.ncapsulator project, TaqI was used along with DpnII to cut up the genetic material within the cell, completing the cell death module.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 18
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 157