Difference between revisions of "Part:BBa K4623010"

 
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<partinfo>BBa_K4623010 short</partinfo>
 
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GFP
 
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===Usage and Biology===
 
===Usage and Biology===
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The initial intent of this part was to visualize the conformational changes in Twisted Silinker (BBa_K46009). For more details, please refer to our wiki. The core of this part is GCaMP6m (BBa_K3755007). When Calmodulin (CaM) responds to calcium ions and binds to Calmodulin Binding Peptide (CBP), the spatial structure of GFP becomes more compact, leading to an increase in fluorescence intensity. We aimed to provide a measurable and quantifiable reference for the challenging-to-measure phenomenon of TS spatial structural changes through the variation in GFP fluorescence intensity. However, the Twisted Silinker (GFP) we ultimately obtained did not exhibit differences in fluorescence intensity at different calcium ion concentrations.
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4623010 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4623010 SequenceAndFeatures</partinfo>
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Revision as of 14:57, 10 October 2023


Twisted Silinker(GFP),The process of protein coiling can be visualized and green fluorescence can be

Usage and Biology

The initial intent of this part was to visualize the conformational changes in Twisted Silinker (BBa_K46009). For more details, please refer to our wiki. The core of this part is GCaMP6m (BBa_K3755007). When Calmodulin (CaM) responds to calcium ions and binds to Calmodulin Binding Peptide (CBP), the spatial structure of GFP becomes more compact, leading to an increase in fluorescence intensity. We aimed to provide a measurable and quantifiable reference for the challenging-to-measure phenomenon of TS spatial structural changes through the variation in GFP fluorescence intensity. However, the Twisted Silinker (GFP) we ultimately obtained did not exhibit differences in fluorescence intensity at different calcium ion concentrations.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1015
    Illegal EcoRI site found at 1543
    Illegal EcoRI site found at 1711
    Illegal EcoRI site found at 1933
    Illegal PstI site found at 1028
    Illegal PstI site found at 1654
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1015
    Illegal EcoRI site found at 1543
    Illegal EcoRI site found at 1711
    Illegal EcoRI site found at 1933
    Illegal PstI site found at 1028
    Illegal PstI site found at 1654
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1015
    Illegal EcoRI site found at 1543
    Illegal EcoRI site found at 1711
    Illegal EcoRI site found at 1933
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1015
    Illegal EcoRI site found at 1543
    Illegal EcoRI site found at 1711
    Illegal EcoRI site found at 1933
    Illegal PstI site found at 1028
    Illegal PstI site found at 1654
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1015
    Illegal EcoRI site found at 1543
    Illegal EcoRI site found at 1711
    Illegal EcoRI site found at 1933
    Illegal PstI site found at 1028
    Illegal PstI site found at 1654
    Illegal AgeI site found at 445
    Illegal AgeI site found at 505
    Illegal AgeI site found at 1234
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1099
    Illegal SapI.rc site found at 1528