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Revision as of 12:41, 10 October 2023


CYP3A4 gene with mStop for Chlamydomonas reinhardtii (Phytobrick)

This composite part contains the AβSAP(i)-promotor (BBa_K4806013), the coding sequence of CYP3A4 (BBa_K4806000), mStop (BBa_K4806009) and the tRPL23-terminator (BBa_K3002006)*. This part is codon-optimized for Chlamydomonas reinhardtii and was built as part of the CYPurify Collection. This level 2 part leads to potential detoxification of specific chemicals (Ohkawa & Inui, 2015).


Construct

Fig.1 Construct design
This construct was designed using the modular cloning system (MoClo).

The resistance cassette for spectinomycin is already built in the level 2 vector pMBS807 we are using. The usage of this vector allows the direct assembly of level 0 parts to level 2 constructs, facilitating the cloning time (Niemeyer & Schroda, 2022).


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1846
    Illegal PstI site found at 2168
    Illegal PstI site found at 2228
    Illegal PstI site found at 2700
    Illegal PstI site found at 2769
    Illegal PstI site found at 2873
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 249
    Illegal PstI site found at 1846
    Illegal PstI site found at 2168
    Illegal PstI site found at 2228
    Illegal PstI site found at 2700
    Illegal PstI site found at 2769
    Illegal PstI site found at 2873
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3178
    Illegal XhoI site found at 530
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1846
    Illegal PstI site found at 2168
    Illegal PstI site found at 2228
    Illegal PstI site found at 2700
    Illegal PstI site found at 2769
    Illegal PstI site found at 2873
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1846
    Illegal PstI site found at 2168
    Illegal PstI site found at 2228
    Illegal PstI site found at 2700
    Illegal PstI site found at 2769
    Illegal PstI site found at 2873
    Illegal NgoMIV site found at 2090
    Illegal NgoMIV site found at 3511
    Illegal AgeI site found at 268
  • 1000
    COMPATIBLE WITH RFC[1000]


Results