Difference between revisions of "Part:BBa K4665120:Design"
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===Source=== | ===Source=== | ||
| − | This part is derived from the genome of extremophilic bacterium <i>Sulfurihydrogenibium azorense</i>. Our sequence is derived from the paper by Zhu et al. | + | This part is derived from the genome of extremophilic bacterium <i>Sulfurihydrogenibium azorense</i>. |
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| + | Our sequence for SazCa is derived from the paper by Zhu et al. (2022), which has been codon-optimized for <i>E. coli BL21(DE3)</i>. | ||
===References=== | ===References=== | ||
Zhu, Y., et.al. (December 6, 2021). Surface display of carbonic anhydrase on Escherichia coli for CO2 capture and mineralisation. Synthetic and Systems biotechnology, 7(1): 460-473. https://doi.org/10.1016%2Fj.synbio.2021.11.008 | Zhu, Y., et.al. (December 6, 2021). Surface display of carbonic anhydrase on Escherichia coli for CO2 capture and mineralisation. Synthetic and Systems biotechnology, 7(1): 460-473. https://doi.org/10.1016%2Fj.synbio.2021.11.008 | ||
Latest revision as of 11:38, 10 October 2023
SazCA Carbonic Anhydrase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This sequence will be attached to a flexible linker sequence at its 5' end for the final construct of our fusion protein
Source
This part is derived from the genome of extremophilic bacterium Sulfurihydrogenibium azorense.
Our sequence for SazCa is derived from the paper by Zhu et al. (2022), which has been codon-optimized for E. coli BL21(DE3).
References
Zhu, Y., et.al. (December 6, 2021). Surface display of carbonic anhydrase on Escherichia coli for CO2 capture and mineralisation. Synthetic and Systems biotechnology, 7(1): 460-473. https://doi.org/10.1016%2Fj.synbio.2021.11.008