Difference between revisions of "Part:BBa K4692000"

 
 
Line 3: Line 3:
 
<partinfo>BBa_K4692000 short</partinfo>
 
<partinfo>BBa_K4692000 short</partinfo>
  
−
This is a protein-coding part, coding for a phytase from Aspergillus japonicus BCC18313. This gene has been codon-optimized for expression in Pichia pastoris. This phytase has been described in an article by Promdonkoy et al. (FEMS Microbiol Lett 2009 290(1):18-24). Optimal phytase activity is at 50 degrees C and pH 5.5. The enzyme is highly thermostable, as it retains >50% activity at 100 degrees C for 10 min. The phytase exhibits broad pH stability from 2.0 to 8.0.
+
This is a protein-coding part, coding for a phytase from Escherichia coli MG1655. This gene has been codon-optimized for expression in Saccharomyces cerevisiae. Optimum temperature is 60 degrees C, and optimum pH is 4.5.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 11:18, 10 October 2023


Phytase from Escherichia coli

This is a protein-coding part, coding for a phytase from Escherichia coli MG1655. This gene has been codon-optimized for expression in Saccharomyces cerevisiae. Optimum temperature is 60 degrees C, and optimum pH is 4.5.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]