Difference between revisions of "Part:BBa K4692001:Design"

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===Design Notes===
 
===Design Notes===
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The DNA sequence has been optimized for expression in the yeast Pichia pastoris. The native secretion signal has been replaced with the alpha mating factor sequence from Saccharomyces cerevisiae. A GS linker has been added between the alpha mating factor sequence and the coding sequence.
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The DNA sequence has been optimized for expression in the yeast Saccharomyces cerevisiae. The native secretion signal has been replaced with the alpha mating factor sequence from Saccharomyces cerevisiae. A GS linker has been added between the alpha mating factor sequence and the coding sequence.
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===Source===
 
===Source===

Latest revision as of 11:03, 10 October 2023


Phytase from Aspergillus japonicus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 679
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The DNA sequence has been optimized for expression in the yeast Saccharomyces cerevisiae. The native secretion signal has been replaced with the alpha mating factor sequence from Saccharomyces cerevisiae. A GS linker has been added between the alpha mating factor sequence and the coding sequence.

Source

This gene is from the filamenteous fungus Aspergillus japonicus BCC18313 (Genbank accession EU786166.1).

References

Promdonkoy P, Tang K, Sornlake W, Harnpicharnchai P, Kobayashi RS, Ruanglek V, Upathanpreecha T, Vesaratchavest M, Eurwilaichitr L, Tanapongpipat S. Expression and characterization of Aspergillus thermostable phytases in Pichia pastoris. FEMS Microbiol Lett. 2009 Jan;290(1):18-24. doi: 10.1111/j.1574-6968.2008.01399.x. Epub 2008 Nov 12. PMID: 19025560.