Difference between revisions of "Part:BBa K4808009"
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<partinfo>BBa_K4808009 short</partinfo>
 
<partinfo>BBa_K4808009 short</partinfo>
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pTarget plasmid that carrying specific gRNA sequence which can identity the target gene for the further gene knockout.
 
pTarget plasmid that carrying specific gRNA sequence which can identity the target gene for the further gene knockout.
  
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<h2><b>Characterization</b></h2>
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    <h2><b>Characterization</b></h2>
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     <p>pTarget plasmid play an important role in our CRISPR-CAS 9 knocking out experiment. </p>
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     <p>pTarget plasmid play an important role in our CRISPR-CAS 9 knocking out experiment. </p >
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    https://static.igem.wiki/teams/4808/wiki/1280x1280.png
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https://static.igem.wiki/teams/4808/wiki/1280x1280.png
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     <p> Step1: Transformation of the pEcCas plasmid(carry cas9 protein) into E. coli AIS-0 (CICC20905). Step2:
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     <p> Step1: Transformation of the pEcCas plasmid(carry cas9 protein) into E. coli AIS-0 (CICC20905). Step2: construction of the pTarget plasmid and donor DNA. Step3: transformation of pTarget plasmid and donor DNA into the AIS-0 with pEsCas inserted. Step4: Incubation of the transformed strains. </p >
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        construction of the pTarget plasmid and donor DNA. Step3: transformation of pTarget plasmid and donor DNA into
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        the AIS-0 with pEsCas inserted. Step4: Incubation of the transformed strains. </p>
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4808009 SequenceAndFeatures</partinfo>
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K4808009 parameters</partinfo>
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<partinfo>BBa_K4808003 parameters</partinfo>
 
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Revision as of 11:02, 10 October 2023

pTarget

pTarget plasmid that carrying specific gRNA sequence which can identity the target gene for the further gene knockout.

Characterization

pTarget plasmid play an important role in our CRISPR-CAS 9 knocking out experiment.

1280x1280.png

Step1: Transformation of the pEcCas plasmid(carry cas9 protein) into E. coli AIS-0 (CICC20905). Step2: construction of the pTarget plasmid and donor DNA. Step3: transformation of pTarget plasmid and donor DNA into the AIS-0 with pEsCas inserted. Step4: Incubation of the transformed strains.