Difference between revisions of "Part:BBa K243003:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | Freiburg standard25 part | + | Freiburg standard25 part.<br> |
+ | [https://parts.igem.org/Image:DigA.txt Commented GenBank file] | ||
===Source=== | ===Source=== |
Revision as of 17:25, 19 October 2009
Digoxigenin binding protein (DigA)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 248
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Freiburg standard25 part.
Commented GenBank file
Source
synthesized by purimex.
References
Holtke HJ, Ankenbauer W, Muhlegger K, Rein R, Sagner G, Seibl R, Walter T. (1995)
The digoxigenin (DIG) system for non-radioactive labelling and detection of nucleic
acids--an overview. Cell Mol Biol. 41(7):883-905.
Roche Applied Science (2002) DIG Application Manual for Nonradioactive In Situ
Hybridization (3rdedition)
Moter A, Göbel UB (2000) Fluorescence in situ hybridisation (FISH) for direct visualization of microorganism, J Microbiol Meth. 41: 85-112
http://www.thermo.com/eThermo/CMA/PDFs/Various/File_29495.pdf (image)