Difference between revisions of "Part:BBa K243003:Design"

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synthesized by purimex.<br>
 
synthesized by purimex.<br>
−
[https://parts.igem.org/Image:DigA.txt  "Commented GenBank file"]
+
[https://parts.igem.org/Image:DigA.txt  Commented GenBank file]
  
 
===References===
 
===References===

Revision as of 17:24, 19 October 2009

Digoxigenin binding protein (DigA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 248
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Freiburg standard25 part

Source

synthesized by purimex.
Commented GenBank file

References

Holtke HJ, Ankenbauer W, Muhlegger K, Rein R, Sagner G, Seibl R, Walter T. (1995)
The digoxigenin (DIG) system for non-radioactive labelling and detection of nucleic acids--an overview. Cell Mol Biol. 41(7):883-905.

Roche Applied Science (2002) DIG Application Manual for Nonradioactive In Situ Hybridization (3rdedition)

Moter A, Göbel UB (2000) Fluorescence in situ hybridisation (FISH) for direct visualization of microorganism, J Microbiol Meth. 41: 85-112

http://www.thermo.com/eThermo/CMA/PDFs/Various/File_29495.pdf (image)