Difference between revisions of "Part:BBa K193000"
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Promoter lambda - cI regulated - (R0051) with GFP (E0040) as a reporter.
 
Promoter lambda - cI regulated - (R0051) with GFP (E0040) as a reporter.
 
This part is used to transform E.coli strain N99,which express cI constitutively, and N4830,which express temperature-sensitive cI constitutively.
 
This part is used to transform E.coli strain N99,which express cI constitutively, and N4830,which express temperature-sensitive cI constitutively.
 +
 +
BBa_S03335 and BBa_J69501 are not available via the Registry distribution.
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 +
Backbone of BBa_K193000 is pSB6A1, but Twins are pSB1 system.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 16:48, 19 October 2009

GFP reporter regulated by CI.

Promoter lambda - cI regulated - (R0051) with GFP (E0040) as a reporter. This part is used to transform E.coli strain N99,which express cI constitutively, and N4830,which express temperature-sensitive cI constitutively.

BBa_S03335 and BBa_J69501 are not available via the Registry distribution.

Backbone of BBa_K193000 is pSB6A1, but Twins are pSB1 system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 719