Difference between revisions of "Part:BBa K4711026"
(→Usage and Biology) |
|||
Line 4: | Line 4: | ||
=Usage and Biology= | =Usage and Biology= | ||
+ | Colony PCR results showed the appearance of the target band at around 4000 bp, indicating successful plasmid integration into Escherichia coli. SDS-PAGE analysis revealed the successful expression of the AlkB, AlkG, and AlkT proteins. Additionally, by setting a gradient of IPTG concentrations, it was observed that the protein expression was most efficient when induced with 0.5 mM IPTG, yielding the highest protein expression levels. | ||
+ | <html> | ||
+ | |||
+ | <figure> | ||
+ | |||
+ | <img src="https://static.igem.wiki/teams/4711/wiki/results/figure-10.png"width="100%" style="float:center"> | ||
+ | |||
+ | <figcaption> | ||
+ | |||
+ | <p style="font-size:1rem"> | ||
+ | Fig 1 (A) Gene circuit (B) Colony PCR (C) SDS-PAGE M: Protein Marker 1: 0mM IPTG 2: 0.2mM IPTG 3: 0.5mM IPTG 4: 1mM IPTG. The protein bands from top to bottom are alkT (56KDa), alkB (45KDa), and alkG (18KDa). | ||
+ | |||
+ | |||
+ | </figcaption> | ||
+ | |||
+ | </figure> | ||
+ | </html> | ||
===Source=== | ===Source=== | ||
Revision as of 09:03, 10 October 2023
T7+alkB+alkG+T7+alkT+Spycatcher
Usage and Biology
Colony PCR results showed the appearance of the target band at around 4000 bp, indicating successful plasmid integration into Escherichia coli. SDS-PAGE analysis revealed the successful expression of the AlkB, AlkG, and AlkT proteins. Additionally, by setting a gradient of IPTG concentrations, it was observed that the protein expression was most efficient when induced with 0.5 mM IPTG, yielding the highest protein expression levels.
Fig 1 (A) Gene circuit (B) Colony PCR (C) SDS-PAGE M: Protein Marker 1: 0mM IPTG 2: 0.2mM IPTG 3: 0.5mM IPTG 4: 1mM IPTG. The protein bands from top to bottom are alkT (56KDa), alkB (45KDa), and alkG (18KDa).
Source
Potential applications
References
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1860
Illegal NheI site found at 3534 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1634
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 167
Illegal NgoMIV site found at 1079
Illegal NgoMIV site found at 2554 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1966