Difference between revisions of "Part:BBa K4743002"
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<center>Figure 2. The Matabolic pathway of NMN production.</center>
 
<center>Figure 2. The Matabolic pathway of NMN production.</center>
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===References===
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1.Fagnocchi, L., Biolchi, A., Ferlicca, F., Boccadifuoco, G., Brunelli, B., Brier, S., Norais, N., Chiarot, E., Bensi, G., Kroll, J. S., Pizza, M., Donnelly, J., Giuliani, M. M., & Delany, I. (2013). Transcriptional regulation of the nadA gene in Neisseria meningitidis impacts the prediction of coverage of a multicomponent meningococcal serogroup B vaccine. Infection and immunity, 81(2), 560–569. https://doi.org/10.1128/IAI.01085-12
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2.Michalowski, C. B., Flachmann, R., Löffelhardt, W., & Bohnert, H. J. (1991). Gene nadA, Encoding Quinolinate Synthetase, Is Located on the Cyanelle DNA from Cyanophora paradoxa. Plant physiology, 95(1), 329–330. https://doi.org/10.1104/pp.95.1.329
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3.Christine B. Michalowski, Ralf Flachmann, Wolfgang Löffelhardt, Hans J. Bohnert, Gene nadA, Encoding Quinolinate Synthetase, Is Located on the Cyanelle DNA from Cyanophora paradoxa  , Plant Physiology, Volume 95, Issue 1, January 1991, Pages 329–330, https://doi.org/10.1104/pp.95.1.329
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 08:54, 10 October 2023


NadA+ADH1 terminator with adapter

NadA gene codes for quinolinate synthase, which turns iminoaspartate into quinolate. The whole pathway is shown below:


NAD biosynthesis, iminoaspartate => quinolinate => NAD

Length checking of nadA

We use electrophoresis to check the length of the fragment

Figure 1. The length of NadA.

Overall metabolic pathway of NMN

NadA is involved in the pathway of Astaxanthin production in PTSH-Taiwan's project.

Figure 2. The Matabolic pathway of NMN production.

References

1.Fagnocchi, L., Biolchi, A., Ferlicca, F., Boccadifuoco, G., Brunelli, B., Brier, S., Norais, N., Chiarot, E., Bensi, G., Kroll, J. S., Pizza, M., Donnelly, J., Giuliani, M. M., & Delany, I. (2013). Transcriptional regulation of the nadA gene in Neisseria meningitidis impacts the prediction of coverage of a multicomponent meningococcal serogroup B vaccine. Infection and immunity, 81(2), 560–569. https://doi.org/10.1128/IAI.01085-12

2.Michalowski, C. B., Flachmann, R., Löffelhardt, W., & Bohnert, H. J. (1991). Gene nadA, Encoding Quinolinate Synthetase, Is Located on the Cyanelle DNA from Cyanophora paradoxa. Plant physiology, 95(1), 329–330. https://doi.org/10.1104/pp.95.1.329

3.Christine B. Michalowski, Ralf Flachmann, Wolfgang Löffelhardt, Hans J. Bohnert, Gene nadA, Encoding Quinolinate Synthetase, Is Located on the Cyanelle DNA from Cyanophora paradoxa , Plant Physiology, Volume 95, Issue 1, January 1991, Pages 329–330, https://doi.org/10.1104/pp.95.1.329

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1284
    Illegal SpeI site found at 1089
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1284
    Illegal SpeI site found at 1089
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1284
    Illegal BamHI site found at 16
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1284
    Illegal SpeI site found at 1089
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1284
    Illegal SpeI site found at 1089
    Illegal AgeI site found at 1297
  • 1000
    COMPATIBLE WITH RFC[1000]