Difference between revisions of "Part:BBa K4613023"

 
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The composite part was constructed to achieve solube expression of ADH3 and analyze the function of ADH3.
 
The composite part was constructed to achieve solube expression of ADH3 and analyze the function of ADH3.
 
The composite part can be directly imported into plasmid and express ADH3 induced with IPTG.
 
The composite part can be directly imported into plasmid and express ADH3 induced with IPTG.
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==== Reference ====
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#Dai L, Niu D, Huang J W, et al. Cryo-EM structure and rational engineering of a superefficient ochratoxin A-detoxifying amidohydrolase[J]. Journal of Hazardous Materials, 2023: 131836.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 08:46, 10 October 2023


pET-46 Ek_LIC-ADH3

The composite part was constructed to achieve solube expression of ADH3 and analyze the function of ADH3. The composite part can be directly imported into plasmid and express ADH3 induced with IPTG.


Reference

  1. Dai L, Niu D, Huang J W, et al. Cryo-EM structure and rational engineering of a superefficient ochratoxin A-detoxifying amidohydrolase[J]. Journal of Hazardous Materials, 2023: 131836.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 827
    Illegal AgeI site found at 515
    Illegal AgeI site found at 677
  • 1000
    COMPATIBLE WITH RFC[1000]