Difference between revisions of "Part:BBa K4759024"

 
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the insufficient supply of heme limits the whole-cell P450 biocatalytic activity.A gene coding for  glutamyl-tRNA reductase (GluTR),Glutamyl-tRNA reductase catalyzes the first step of porphyrin biosynthesis.
 
the insufficient supply of heme limits the whole-cell P450 biocatalytic activity.A gene coding for  glutamyl-tRNA reductase (GluTR),Glutamyl-tRNA reductase catalyzes the first step of porphyrin biosynthesis.
  
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<!-- Add more about the biology of this part here
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===Design===
 +
Recombinant plasmid pETDuet-hemB-hemC-hemD-hemH is expressed in engineered bacteria
 +
 
 
===Usage and Biology===
 
===Usage and Biology===
  

Revision as of 08:19, 10 October 2023


hemH

the insufficient supply of heme limits the whole-cell P450 biocatalytic activity.A gene coding for glutamyl-tRNA reductase (GluTR),Glutamyl-tRNA reductase catalyzes the first step of porphyrin biosynthesis.


Design

Recombinant plasmid pETDuet-hemB-hemC-hemD-hemH is expressed in engineered bacteria

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 871
    Illegal AgeI site found at 16
  • 1000
    COMPATIBLE WITH RFC[1000]