Difference between revisions of "Part:BBa K4759022"
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The enzyme works by stepwise addition of pyrrolylmethyl groups until a hexapyrrole is present at the active center. The terminal tetrapyrrole is then hydrolyzed to yield the product, leaving a cysteine-bound dipyrrole on which assembly continues. | The enzyme works by stepwise addition of pyrrolylmethyl groups until a hexapyrrole is present at the active center. The terminal tetrapyrrole is then hydrolyzed to yield the product, leaving a cysteine-bound dipyrrole on which assembly continues. | ||
H2O + 4 porphobilinogen <=> hydroxymethylbilane + 4 NH4(+) | H2O + 4 porphobilinogen <=> hydroxymethylbilane + 4 NH4(+) | ||
| + | |||
| + | ===Design=== | ||
| + | Recombinant plasmid pETDuet-hemB-hemC-hemD-hemH is expressed in engineered bacteria | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 07:51, 10 October 2023
hemC
The insufficient supply of heme limits the whole-cell P450 biocatalytic activity.A gene coding for glutamyl-tRNA reductase (GluTR),Glutamyl-tRNA reductase catalyzes the first step of porphyrin biosynthesis.A gene coding for porphobilinogen deaminase (PBGD) The enzyme works by stepwise addition of pyrrolylmethyl groups until a hexapyrrole is present at the active center. The terminal tetrapyrrole is then hydrolyzed to yield the product, leaving a cysteine-bound dipyrrole on which assembly continues. H2O + 4 porphobilinogen <=> hydroxymethylbilane + 4 NH4(+)
Design
Recombinant plasmid pETDuet-hemB-hemC-hemD-hemH is expressed in engineered bacteria
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 262
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 262
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 262
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 262
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 262
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 874