Difference between revisions of "Part:BBa K4711012"

 
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Miscellaneous
 
Miscellaneous
 
Unlike other rubredoxins, this rubredoxin is unique in that it contains two binding sites for iron. It is most probably the product of a gene duplication event. The 2Fe form of the rubredoxin is a physiological form but it is less stable than the readily isolated 1Fe form. Both domains can form productive electron transfer complexes with rubredoxin reductase and accept electrons from the enzyme bound FAD.
 
Unlike other rubredoxins, this rubredoxin is unique in that it contains two binding sites for iron. It is most probably the product of a gene duplication event. The 2Fe form of the rubredoxin is a physiological form but it is less stable than the readily isolated 1Fe form. Both domains can form productive electron transfer complexes with rubredoxin reductase and accept electrons from the enzyme bound FAD.
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      <img src="https://static.igem.wiki/teams/4711/wiki/part-collection/pw9t-lfplb6vk-e-9500-pk.png"width="100%" style="float:center">
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Fig 1 The 3D structure of alkB
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===Source===
 
===Source===

Latest revision as of 07:29, 10 October 2023


Rubredoxin-2 (alkG)

Usage and Biology

Function Involved in the hydrocarbon hydroxylating system, which transfers electrons from NADH to rubredoxin reductase and then through rubredoxin to alkane 1 monooxygenase.2 Publications

Miscellaneous Unlike other rubredoxins, this rubredoxin is unique in that it contains two binding sites for iron. It is most probably the product of a gene duplication event. The 2Fe form of the rubredoxin is a physiological form but it is less stable than the readily isolated 1Fe form. Both domains can form productive electron transfer complexes with rubredoxin reductase and accept electrons from the enzyme bound FAD.

Fig 1 The 3D structure of alkB

Source

Organism:Pseudomonas oleovorans

References

[1]Kok M, Oldenhuis R, van der Linden MP, Meulenberg CH, Kingma J, Witholt B. The Pseudomonas oleovorans alkBAC operon encodes two structurally related rubredoxins and an aldehyde dehydrogenase. J Biol Chem. 1989 Apr 5;264(10):5442-51. PMID: 2647719. [2]Benson A, Tomoda K, Chang J, Matsueda G, Lode ET, Coon MJ, Yasunobu KT. Evolutionary and phylogenetic relationships of rubredoxin-containing microbes. Biochem Biophys Res Commun. 1971 Feb 19;42(4):640-6. doi: 10.1016/0006-291x(71)90536-5. PMID: 5543946.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 303
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]