Difference between revisions of "Part:BBa K177047"
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<partinfo>BBa_K177047 short</partinfo>
 
<partinfo>BBa_K177047 short</partinfo>
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Derivative of OR3
  
  
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This variant of the cro binding box differs from the OR1 ([https://parts.igem.org/wiki/index.php/Part:BBa_K177002 BBa_K177002]) version by binding strength to Cro and λ repressor, as indicated by the free energy change, being -1.1 kcal/mol and 2.2 kcal/mol respectively.<small><sup>1</sup></small>
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This variant of the cro binding box differs from the "wild-type" ([https://parts.igem.org/wiki/index.php/Part:BBa_K177002 BBa_K177002]) version by binding strength to Cro and λ repressor, as indicated by the free energy change, being -1.1 kcal/mol and 2.2 kcal/mol respectively.<small><sup>1</sup></small>
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Revision as of 16:15, 19 October 2009

crobox alternative version Derivative of OR3


This variant of the cro binding box differs from the OR1 (BBa_K177002) version by binding strength to Cro and λ repressor, as indicated by the free energy change, being -1.1 kcal/mol and 2.2 kcal/mol respectively.1


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


1 Takeda Y., Sarai A., Rivera VM.; Analysis of the sequence-specific interactions between Cro repressor and operator DNA bt systematic base substitution experiments; Proc. Natl. Acad. Sci. USA, Vol. 86, pp. 439-443, January 1989