Difference between revisions of "Part:BBa K4711021"

 
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=Usage and Biology=
 
=Usage and Biology=
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SpyCatcher is a peptide that forms a spontaneous amide bond with its protein partner SpyTag. This protein superglue is a broadly useful tool for molecular assembly, locking together biological building blocks efficiently and irreversibly in diverse architectures.
  
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  <figure>
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      <img src="https://static.igem.wiki/teams/4711/wiki/part-collection/dv-lysu-aq9i5kv3u495-nl.png"width="100%" style="float:center">
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      <figcaption>
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      <p style="font-size:1rem">
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Fig 1 SpyCatcher-SpyTag three-dimensional structure
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      </figcaption>
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  </figure>
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===Source===
 
===Source===
  
 
===References===
 
===References===
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[1]Keeble, Anthony H ,Banerjee,et al.Evolving Accelerated Amidation by SpyTag/SpyCatcher to Analyze Membrane Dynamics.[J].Angewandte Chemie, 2017.DOI:10.1002/ange.201707623.
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[2]Zakeri B , Fierer J O , Celik E ,et al.Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin[J].Proceedings of the National Academy of Sciences of the United States of America, 2012(12).DOI:10.1073/PNAS.1115485109.
  
 
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Latest revision as of 07:21, 10 October 2023


Spycatcher

Usage and Biology

SpyCatcher is a peptide that forms a spontaneous amide bond with its protein partner SpyTag. This protein superglue is a broadly useful tool for molecular assembly, locking together biological building blocks efficiently and irreversibly in diverse architectures.

Fig 1 SpyCatcher-SpyTag three-dimensional structure

Source

References

[1]Keeble, Anthony H ,Banerjee,et al.Evolving Accelerated Amidation by SpyTag/SpyCatcher to Analyze Membrane Dynamics.[J].Angewandte Chemie, 2017.DOI:10.1002/ange.201707623.

[2]Zakeri B , Fierer J O , Celik E ,et al.Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin[J].Proceedings of the National Academy of Sciences of the United States of America, 2012(12).DOI:10.1073/PNAS.1115485109.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]