Difference between revisions of "Part:BBa K4885012:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | 1 | + | We designed Ptkt-adhE2 to improve the part Pthl-adhE2. Clostridium tyrobutyricum (C. tyrobutyricum) transfected with the plasmid containing Pthl-adhE2 had the capacity to produce butanol in addition to its native capacity of butyrate synthesis, which can be used to manufacture butyl butyrate by lipase catalyzed esterification. For the esterification reaction, the optimal butyrate-to butanol molar ratio is 1:1, however this strain produced far more butanol than butyrate. Here, we used a weaker promoter Ptkt to express adhE2 to lower the yield of butanol and increase the yield of butyrate in the strain, so the engineered strain could achieve a better production ratio of the two precursors for butyl butyrate synthesis. |
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===Source=== | ===Source=== | ||
Revision as of 07:19, 10 October 2023
Ptkt-adhE2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We designed Ptkt-adhE2 to improve the part Pthl-adhE2. Clostridium tyrobutyricum (C. tyrobutyricum) transfected with the plasmid containing Pthl-adhE2 had the capacity to produce butanol in addition to its native capacity of butyrate synthesis, which can be used to manufacture butyl butyrate by lipase catalyzed esterification. For the esterification reaction, the optimal butyrate-to butanol molar ratio is 1:1, however this strain produced far more butanol than butyrate. Here, we used a weaker promoter Ptkt to express adhE2 to lower the yield of butanol and increase the yield of butyrate in the strain, so the engineered strain could achieve a better production ratio of the two precursors for butyl butyrate synthesis.
Source
1