Difference between revisions of "Part:BBa K4711022"
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===References=== | ===References=== | ||
| + | [1]Keeble, Anthony H ,Banerjee,et al.Evolving Accelerated Amidation by SpyTag/SpyCatcher to Analyze Membrane Dynamics.[J].Angewandte Chemie, 2017.DOI:10.1002/ange.201707623. | ||
| + | [2]Zakeri B , Fierer J O , Celik E ,et al.Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin[J].Proceedings of the National Academy of Sciences of the United States of America, 2012(12).DOI:10.1073/PNAS.1115485109. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 07:19, 10 October 2023
SpyTag
Usage and Biology
SpyTag is a peptide that forms a spontaneous amide bond with its protein partner SpyCatcher. This protein superglue is a broadly useful tool for molecular assembly, locking together biological building blocks efficiently and irreversibly in diverse architectures.
Fig 1 SpyCatcher-SpyTag three-dimensional structure
Source
References
[1]Keeble, Anthony H ,Banerjee,et al.Evolving Accelerated Amidation by SpyTag/SpyCatcher to Analyze Membrane Dynamics.[J].Angewandte Chemie, 2017.DOI:10.1002/ange.201707623.
[2]Zakeri B , Fierer J O , Celik E ,et al.Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin[J].Proceedings of the National Academy of Sciences of the United States of America, 2012(12).DOI:10.1073/PNAS.1115485109. Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]