Difference between revisions of "Part:BBa K4765134"

 
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<partinfo>BBa_K4765134 short</partinfo>
 
<partinfo>BBa_K4765134 short</partinfo>
  
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ribozyme connected: Dsup + FEN1 + H. ex mtSSB + SAHS 33020 + XRCC1
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<html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/4765/wiki/2023-b-home.png" alt="contributed by Fudan iGEM 2023"></html>
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===Usage and Biology===
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This part is the combination of all the anti-desiccation and anti-UV proteins, we've transformed it to ''E .coli'' BL21 DE3 and test its performance.
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===Characterization===
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 07:17, 10 October 2023


ribozyme connected: Dsup + FEN1 + H. ex mtSSB + SAHS 33020 + XRCC1

contributed by Fudan iGEM 2023

Usage and Biology

This part is the combination of all the anti-desiccation and anti-UV proteins, we've transformed it to E .coli BL21 DE3 and test its performance.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4573
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1886
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2464
    Illegal NgoMIV site found at 2782
    Illegal AgeI site found at 230
    Illegal AgeI site found at 301
    Illegal AgeI site found at 1570
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3415
    Illegal BsaI.rc site found at 227
    Illegal BsaI.rc site found at 3050
    Illegal BsaI.rc site found at 3131
    Illegal BsaI.rc site found at 5623
    Illegal SapI site found at 2944
    Illegal SapI.rc site found at 956