Difference between revisions of "Part:BBa K4759025"

 
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(S)-4-amino-5-oxopentanoate <=> 5-aminolevulinate
 
(S)-4-amino-5-oxopentanoate <=> 5-aminolevulinate
  
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<!-- Add more about the biology of this part here
+
===Design Notes===
 +
Recombinant plasmid pCDFDuet-hemA-hemL is expressed in engineered bacteria
 +
 
 
===Usage and Biology===
 
===Usage and Biology===
  

Revision as of 07:05, 10 October 2023


hemL

the insufficient supply of heme limits the whole-cell P450 biocatalytic activity.A gene coding for glutamyl-tRNA reductase (GluTR),Glutamyl-tRNA reductase catalyzes the first step of porphyrin biosynthesis.Glutamate-1-semialdehyde aminotransferase catalyzes the pyridoxal 5'-phosphate-dependent reaction which converts GSA to delta-aminolevulinate (ALA). (S)-4-amino-5-oxopentanoate <=> 5-aminolevulinate

Design Notes

Recombinant plasmid pCDFDuet-hemA-hemL is expressed in engineered bacteria

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1028
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1028
    Illegal NheI site found at 738
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1028
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1028
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1028
    Illegal AgeI site found at 388
    Illegal AgeI site found at 721
  • 1000
    COMPATIBLE WITH RFC[1000]