Difference between revisions of "Part:BBa K4630200:Design"

 
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===References===
 
===References===
 +
Zhao, D., Yuan, S., Xiong, B. et al. Development of a fast and easy method for Escherichia coli genome editing with CRISPR/Cas9. Microb Cell Fact 15, 205 (2016).

Latest revision as of 07:02, 10 October 2023


plasmid pCas for expressing Cas9 and Lambda Red recombinases


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1344
    Illegal SpeI site found at 5563
    Illegal PstI site found at 11241
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1344
    Illegal NheI site found at 1103
    Illegal SpeI site found at 5563
    Illegal PstI site found at 11241
    Illegal NotI site found at 5882
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1344
    Illegal BglII site found at 4205
    Illegal BamHI site found at 3382
    Illegal BamHI site found at 7647
    Illegal BamHI site found at 11830
    Illegal XhoI site found at 4323
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1344
    Illegal SpeI site found at 5563
    Illegal PstI site found at 11241
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1344
    Illegal SpeI site found at 5563
    Illegal PstI site found at 11241
    Illegal NgoMIV site found at 10790
    Illegal AgeI site found at 7482
    Illegal AgeI site found at 9139
    Illegal AgeI site found at 11665
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 7464
    Illegal SapI site found at 10730
    Illegal SapI site found at 10940
    Illegal SapI site found at 11647


Design Notes

The plasmid is temperature sensitive plasmid.


Source

Zhao, D., Yuan, S., Xiong, B. et al. Development of a fast and easy method for Escherichia coli genome editing with CRISPR/Cas9. Microb Cell Fact 15, 205 (2016).

References

Zhao, D., Yuan, S., Xiong, B. et al. Development of a fast and easy method for Escherichia coli genome editing with CRISPR/Cas9. Microb Cell Fact 15, 205 (2016).