Difference between revisions of "Part:BBa K4759201"
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<partinfo>BBa_K4759201 short</partinfo> | <partinfo>BBa_K4759201 short</partinfo> | ||
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ferredoxin reductase CamA and ferredoxin CamB are selected as redox partner. | ferredoxin reductase CamA and ferredoxin CamB are selected as redox partner. | ||
===Design === | ===Design === | ||
Revision as of 06:59, 10 October 2023
T7-RBS1-camA-RBS2-camB
ferredoxin reductase CamA and ferredoxin CamB are selected as redox partner.
Design
codon-optimized
Usage and Biology
the CYP107 family is a typical three-component P450 enzyme that requires a pair of redox partner to transfer electrons from the electron donor NAD(P)H to the P450 enzyme activity center, ferredoxin reductase CamA and ferredoxin CamB are selected as redox partner. The codon-optimized camA gene and camB gene are synthesized, and they are subcloned to the plasmid pACYCDuet to obtain the recombinant plasmid pACYCDuet-camA-camB Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1366
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1366
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1366
Illegal BamHI site found at 1360 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1366
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1366
Illegal AgeI site found at 124
Illegal AgeI site found at 418 - 1000COMPATIBLE WITH RFC[1000]