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Latest revision as of 06:33, 10 October 2023
NeuAc riboswitch
Usage and biology
In order to prevent E. coli from leaking into the environment, we designed the NeuAc ribose switch. The NeuAc ribose switch consists of an RNA aptamer and a hammerhead ribozyme. The principle of its action is that NeuAc binding to the RNA aptamer causes the conformational change of the entire switch, resulting in hammerhead ribozyme self-shearing, resulting in a notch, and then under the action of ReJ ribozyme in Escherichia coli cells, the following genes are cut, thereby reducing the abundance of mRNA expressed downstream genes. In order to ensure that the switch can work effectively, there are spacers at both ends of the switch, and the 5' end is 5-AAAAATAAAAAGAAAA-3 , and the 3 'end is 5-AAAAAACAAAA-3.
Fig 1 NeuAc Riboswitch Structure
Fig 2 Schematic diagram of the riboswitch constructed in this experiment
Source
Potential applications
References
[1]Suhyung,Cho,Bo-Rahm,et al.In vitro selection of sialic acid specific RNA aptamer and its application to the rapid sensing of sialic acid modified sugars[J].Biotechnology & Bioengineering, 2012.DOI:10.1002/bit.24737.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]