Difference between revisions of "Part:BBa K177047"
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<partinfo>BBa_K177047 short</partinfo> | <partinfo>BBa_K177047 short</partinfo> | ||
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| + | This variant of the cro binding box differs from the "wild-type" ([https://parts.igem.org/wiki/index.php/Part:BBa_K177002 BBa_K177002]) version by binding strength to Cro and λ repressor, as indicated by the free energy change, being -1.1 kcal/mol and 2.2 kcal/mol respectively.<small><sup>1</sup></small> | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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<partinfo>BBa_K177047 SequenceAndFeatures</partinfo> | <partinfo>BBa_K177047 SequenceAndFeatures</partinfo> | ||
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| + | <small><sup>1</sup></small> Takeda Y., Sarai A., Rivera VM.; Analysis of the sequence-specific interactions between Cro repressor and operator DNA bt systematic base substitution experiments; Proc. Natl. Acad. Sci. USA, Vol. 86, pp. 439-443, January 1989 | ||
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Revision as of 15:56, 19 October 2009
crobox alternative version
This variant of the cro binding box differs from the "wild-type" (BBa_K177002) version by binding strength to Cro and λ repressor, as indicated by the free energy change, being -1.1 kcal/mol and 2.2 kcal/mol respectively.1
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
1 Takeda Y., Sarai A., Rivera VM.; Analysis of the sequence-specific interactions between Cro repressor and operator DNA bt systematic base substitution experiments; Proc. Natl. Acad. Sci. USA, Vol. 86, pp. 439-443, January 1989