Difference between revisions of "Part:BBa K4630001:Design"

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===References===
 
===References===
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1. Perli SD, Cui CH, Lu TK. Continuous genetic recording with self-targeting CRISPR-Cas in human cells. Science. 2016;353(6304):aag0511. doi:10.1126/science.aag0511

Latest revision as of 05:33, 10 October 2023


self-targeting guide RNA 1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The stgRNA 1 serves for self-targeting with high efficiency. First, we designed the SDS using our own N20 generator (see Model). Also, the sgRNA scaffold downstream the SDS sequence was modified to "GGG", which gives a PAM for the knock-out.

Source

Complete synthesis.


References

1. Perli SD, Cui CH, Lu TK. Continuous genetic recording with self-targeting CRISPR-Cas in human cells. Science. 2016;353(6304):aag0511. doi:10.1126/science.aag0511