Difference between revisions of "Part:BBa K4630001:Design"

 
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===Design Notes===
 
===Design Notes===
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The stgRNA 1 serves for self-targeting with high efficiency. First, we designed the SDS using our own N20 generator (see Model). Also, the sgRNA scaffold right after the SDS sequence was modified to "GGG", which gives a PAM for the knock-out.
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The stgRNA 1 serves for self-targeting with high efficiency. First, we designed the SDS using our own N20 generator (see Model). Also, the sgRNA scaffold downstream the SDS sequence was modified to "GGG", which gives a PAM for the knock-out.
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===Source===
 
===Source===

Revision as of 05:31, 10 October 2023


self-targeting guide RNA 1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The stgRNA 1 serves for self-targeting with high efficiency. First, we designed the SDS using our own N20 generator (see Model). Also, the sgRNA scaffold downstream the SDS sequence was modified to "GGG", which gives a PAM for the knock-out.

Source

Complete synthesis.


References