Difference between revisions of "Part:BBa K4156083:Design"
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'''Group''': [https://2023.igem.wiki/ouc-china/parts iGEM Team OUC-China 2023] | '''Group''': [https://2023.igem.wiki/ouc-china/parts iGEM Team OUC-China 2023] | ||
| − | '''Author''': | + | '''Author''': Qingyu Wu |
'''Summary''': Successfully demonstrated the lysis function of this gene | '''Summary''': Successfully demonstrated the lysis function of this gene | ||
Latest revision as of 05:26, 10 October 2023
φ174E
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
It is important to note that φ174E does not exist in the igem whitelist. We have drafted a safety precaution about φ174E and submitted the check-in form to igem official. The details can be seen on our wiki.
Demonstration of the function of the lysis gene
Group: iGEM Team OUC-China 2023
Author: Qingyu Wu
Summary: Successfully demonstrated the lysis function of this gene
After induction with IPTG for 2-3 hours, the lysin protein can almost completely kill all bacteria. According to the results of SDS-PAGE in the figure below, it can be observed that there are almost no protein bands in the induced group. This is because a large amount of protease is released after cell death, leading to protein degradation, indicating that the lysin protein is functioning.
Fig 1 (A) Gene circuit (B) Colony PCR (C) SDS-PAGE M: Protein Marker 1: 0mM IPTG 2: 0.2mM IPTG
Source
φ174E is a lysis gene from phage Φx174.