Difference between revisions of "Part:BBa K4743034"

Revision as of 01:37, 10 October 2023

Astaxanthin production set

This set is used for Astaxanthin to produced in Kluryveromyces Marxianus

Overall metabolic pathway of Astaxanthin

Figure 1. The Matabolic pathway of Astaxanthin production.

Phenotyping

Figure 2. The phenotyping of our strain for the third generation.

Quantitative check

Figure 3. The HPLC test for the third generation.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 8176
Illegal PstI site found at 8266
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 8176
Illegal NheI site found at 627
Illegal NheI site found at 915
Illegal NheI site found at 1119
Illegal NheI site found at 2274
Illegal NheI site found at 9773
Illegal NheI site found at 10061
Illegal NheI site found at 10265
Illegal PstI site found at 8266
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 8176
Illegal BglII site found at 725
Illegal BglII site found at 5611
Illegal BglII site found at 9871
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 8176
Illegal PstI site found at 8266
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 8176
Illegal PstI site found at 8266
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 4377
Illegal BsaI.rc site found at 12941

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 <html><img style="width:800px" src="https://static.igem.wiki/teams/4743/wiki/folder-for-parts/astaxanthin-hplc.png" </html>
 <html><img style="width:300px" src="https://static.igem.wiki/teams/4743/wiki/folder-for-parts/astaxanthin-quantitative-data.png" </html>
 <center>Figure 3. The HPLC test for the third generation. </center>