Difference between revisions of "Part:BBa K5023012"

Revision as of 01:28, 10 October 2023

PAR_Ble_SP7_FAST-PETase_Linker_MHETase_SP20_Ter

This composite part have: PAR promoter, the bleomycin/zeocin resistance sequence, signal peptide, the Fast-PETase linked to MHETase, Tag, and the terminator. Designed to express and secrete Fast-PETase linked to MHETase in Chlamydomonas reinhardtii, utilizing pJP32 backbone.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 704
Illegal PstI site found at 2129
Illegal PstI site found at 2920
Illegal PstI site found at 3298
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 249
Illegal NheI site found at 1223
Illegal PstI site found at 704
Illegal PstI site found at 2129
Illegal PstI site found at 2920
Illegal PstI site found at 3298
Illegal NotI site found at 3309
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 1634
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 704
Illegal PstI site found at 2129
Illegal PstI site found at 2920
Illegal PstI site found at 3298
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 704
Illegal PstI site found at 2129
Illegal PstI site found at 2920
Illegal PstI site found at 3298
Illegal NgoMIV site found at 1085
Illegal NgoMIV site found at 1146
Illegal NgoMIV site found at 1438
Illegal NgoMIV site found at 3236
Illegal NgoMIV site found at 3368
Illegal NgoMIV site found at 3398
Illegal NgoMIV site found at 3965
1000
COMPATIBLE WITH RFC[1000]

Revision as of 21:14, 8 October 2023 (view source) Registry (Talk \| contribs)		Revision as of 01:28, 10 October 2023 (view source) Lucasovelar (Talk \| contribs) Newer edit →
Line 3:		Line 3:
	<partinfo>BBa_K5023012 short</partinfo>		<partinfo>BBa_K5023012 short</partinfo>

−	~~With the~~ PAR promoter, the bleomycin/zeocin resistance sequence, signal peptide, the Fast-PETase linked to MHETase, Tag, and the terminator.	+	This composite part have: PAR promoter, the bleomycin/zeocin resistance sequence, signal peptide, the Fast-PETase linked to MHETase, Tag, and the terminator. Designed to express and secrete Fast-PETase linked to MHETase in Chlamydomonas reinhardtii, utilizing pJP32 backbone.
−		+