Difference between revisions of "Part:BBa K4759203"

 
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===Usage and Biology===
 
===Usage and Biology===
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OleP, the cytochrome P450 epoxidase from Streptomyces antibioticus involved in Oleandomycin biosynthesis: functional analysis and crystallographic structure in complex with clotrimazole.
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PDB DOI:  https://doi.org/10.2210/pdb4XE3/pdb
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Classification: OXIDOREDUCTASE
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Organism(s): Streptomyces antibioticus
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Expression System: Escherichia coli BL21(DE3)
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https://static.igem.wiki/teams/4759/wiki/4xe3.png
  
 
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Revision as of 01:24, 10 October 2023


T7-RBS4-OleP

we selected high-copy plasmids pRSFDuet, medium-high copy plasmids pETDuet and pET28a, and low-copy plasmid pACYCDuet as carriers to express Olep in E. coli, and the results showed that the recombinant strains constructed with high-copy plasmid pRSFDuet as the carrier had the highest expression of p450 enzyme, cell growth rate, and substrate conversion rate.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 102
    Illegal AgeI site found at 200
  • 1000
    COMPATIBLE WITH RFC[1000]