Difference between revisions of "Part:BBa K4759202"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | OleP, the cytochrome P450 epoxidase from Streptomyces antibioticus involved in Oleandomycin biosynthesis: functional analysis and crystallographic structure in complex with clotrimazole. | ||
| + | PDB DOI: https://doi.org/10.2210/pdb4XE3/pdb | ||
| + | Classification: OXIDOREDUCTASE | ||
| + | Organism(s): Streptomyces antibioticus | ||
| + | Expression System: Escherichia coli BL21(DE3) | ||
| + | |||
| + | https://static.igem.wiki/teams/4759/wiki/4xe3.png | ||
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Revision as of 01:23, 10 October 2023
T7-RBS3-OleP
P450 enzyme CYP107D1 derived from Streptomyces, abbreviated as Olep, is selected to synthesize the above gene by adding histidine tags at the C-terminus through E. coli codon optimization and subcloning the above genes onto the plasmid pET-28a to obtain the recombinant plasmid pET-28a-oleP.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 47
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 47
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 47
Illegal AgeI site found at 103
Illegal AgeI site found at 201 - 1000COMPATIBLE WITH RFC[1000]