Difference between revisions of "Part:BBa K203118"
(→‎Functional Parameters)
 
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<partinfo>BBa_K203118 parameters</partinfo>
 
<partinfo>BBa_K203118 parameters</partinfo>
  
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We generated a library of constitutive promoters by [http://2009.igem.org/Team:Heidelberg/Project_Synthetic_promoters RA-PCR]. We characterized those promoters by Flow cytometry.
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We generated a library of constitutive promoters by [http://2009.igem.org/Team:Heidelberg/Project_Synthetic_promoters RA-PCR]. We characterized those promoters by Flow cytometry in [[Part:BBa_K203100]].
  
 
[[Image:HD09_constitutive.png]]
 
[[Image:HD09_constitutive.png]]

Latest revision as of 13:47, 19 October 2009

Constitutive promoter; 1 REU

A constitutive promoter from the Heidelberg 2009 library of promoters for use in mammalian cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

We generated a library of constitutive promoters by [http://2009.igem.org/Team:Heidelberg/Project_Synthetic_promoters RA-PCR]. We characterized those promoters by Flow cytometry in Part:BBa_K203100.

HD09 constitutive.png

This promoter corresponds to clone L1. We confirmed the initial measurement by an independet technique (image analysis). Its expression strength is approx 1 [http://2009.igem.org/Team:Heidelberg/Project_Measurement REU].