Difference between revisions of "Part:BBa K4719023"
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<partinfo>BBa_K4719023 short</partinfo> | <partinfo>BBa_K4719023 short</partinfo> | ||
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| + | <body> | ||
| + | <h2>Introduction</h2> | ||
| + | <p> | ||
| + | <b>Vilnius-Lithuania iGEM 2023</b> team's goal was to create <b> synthetic biology tools for <i>in vivo</i> alterations of <i>Komagataeibacter xylinus</i> bacterial cellulose polymer composition</b>. Firstly, we chose to produce a <b>cellulose-chitin copolymer</b> that would later be deacetylated, creating <b>bacterial cellulose-chitosan</b>. This polymer is an easily modifiable platform when compared to bacterial cellulose. The enhanced chemical reactivity of the bacterial cellulose-chitosan polymer allows for specific functionalizations in the biomedicine field, such as scaffold design. As a second approach, we designed <b>indigo-dyed cellulose</b> that could be used as a green chemistry way to apply cellulose in the textile industry. Lastly, we have achieved a of <b>bacterial cellulose and polyhydroxybutyrate (PHB) composite</b>, which is synthesized by <i>K. xylinus</i>. | ||
| + | </p> | ||
| − | The FRF-linker is constituted of three parts: 2 flexible ends and a rigid middle section. The initial version of this linker was generated with our software and was later curated manually. The graph for the flexibility profile is listed below. | + | <h2>Usage and Biology</h2> |
| + | This is a synthetic linker generated by Vilnius-Lithuania 2023 software. The FRF-linker is constituted of three parts: 2 flexible ends and a rigid middle section. The initial version of this linker was generated with our software and was later curated manually. The graph for the flexibility profile is listed below. | ||
| + | <figure> | ||
| + | <div class = "center" > | ||
| + | <center><img src = "https://static.igem.wiki/teams/4719/wiki/partai/frf-dfi.png" style = "width:400px;"></center> | ||
| + | </div> | ||
| + | <figcaption><center><b>Figure 1:</b> Graph of linker rigidity, low DFI means ridig section, while high DFI is flexible section.</center></figcaption> | ||
| + | </figure> | ||
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Revision as of 21:05, 9 October 2023
Flexible Rigid Flexible (FRF) linker
Introduction
Vilnius-Lithuania iGEM 2023 team's goal was to create synthetic biology tools for in vivo alterations of Komagataeibacter xylinus bacterial cellulose polymer composition. Firstly, we chose to produce a cellulose-chitin copolymer that would later be deacetylated, creating bacterial cellulose-chitosan. This polymer is an easily modifiable platform when compared to bacterial cellulose. The enhanced chemical reactivity of the bacterial cellulose-chitosan polymer allows for specific functionalizations in the biomedicine field, such as scaffold design. As a second approach, we designed indigo-dyed cellulose that could be used as a green chemistry way to apply cellulose in the textile industry. Lastly, we have achieved a of bacterial cellulose and polyhydroxybutyrate (PHB) composite, which is synthesized by K. xylinus.
Usage and Biology
This is a synthetic linker generated by Vilnius-Lithuania 2023 software. The FRF-linker is constituted of three parts: 2 flexible ends and a rigid middle section. The initial version of this linker was generated with our software and was later curated manually. The graph for the flexibility profile is listed below.