Difference between revisions of "Part:BBa K4613888:Design"

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<em>Agrobacterium tumefaciens</em> R10
 
<em>Agrobacterium tumefaciens</em> R10
  
−
<em>phage wX174</em>
+
phage wX174
  
 
The RBS in <em>Ptra</em> is from the <em>LuxI/LuxR</em> quorum sensing system.
 
The RBS in <em>Ptra</em> is from the <em>LuxI/LuxR</em> quorum sensing system.

Latest revision as of 20:26, 9 October 2023


pSB1C3-tra-lysis


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 429
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 429
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 429
    Illegal BglII site found at 14
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 429
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 429
    Illegal NgoMIV site found at 1592
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We assembled this composite part together by using In-Fusion Cloning. When designing primers, care should be taken because the sequences before Lysis E and traI are identical.


Source

Agrobacterium tumefaciens R10

phage wX174

The RBS in Ptra is from the LuxI/LuxR quorum sensing system.

Reference

  1. White C E, Winans S C.Identification of amino acid residues of the Agrobacterium tumefaciens quorum-sensing regulator TraR that are critical for positive control of transcription[J].Mol Microbiol,2005, 55 (5): 1473-86.
  2. Fuqua C, Winans S C.Conserved cis-acting promoter elements are required for density-dependent transcription of Agrobacterium tumefaciens conjugal transfer genes[J].J Bacteriol,1996, 178 (2): 435-40.
  3. White C E, Winans S C.The quorum-sensing transcription factor TraR decodes its DNA binding site by direct contacts with DNA bases and by detection of DNA flexibility[J].Mol Microbiol,2007, 64 (1): 245-56.
  4. Henrich B, Lubitz W, Plapp R.Lysis of Escherichia coli by induction of cloned phi X174 genes[J].Mol Gen Genet,1982, 185 (3): 493-7.

Young K D, Young R.Lytic action of cloned phi X174 gene E[J].J Virol,1982, 44 (3): 993-1002.

  1. Bernhardt T G, Roof W D, Young R.Genetic evidence that the bacteriophage phi X174 lysis protein inhibits cell wall synthesis[J].Proc Natl Acad Sci U S A,2000, 97 (8): 4297-302.