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===References===
===References===
Revision as of 20:16, 9 October 2023
pSB1C3-tra-lysis
Assembly Compatibility:
10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 429
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 429
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 429 Illegal BglII site found at 14
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 429
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 429 Illegal NgoMIV site found at 1592
1000
COMPATIBLE WITH RFC[1000]
Design Notes
We assembled this composite part together by using In-Fusion Cloning. When designing primers, care should be taken because the sequences before Lysis E and traI are identical.
