Difference between revisions of "Part:BBa K216004"
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<partinfo>BBa_K216004 short</partinfo> | <partinfo>BBa_K216004 short</partinfo> | ||
− | Trz hybrid signal transduction protein: this was created by fusing the extracellular receptor domain of the chemoreceptor Trg with the intracellular signal transduction domain of 2-component sensor protein EnvZ, which normally phosphorylates response regulator OmpR (see Baumgartner, J.W., Kim, C., Brissette, R.E., Inouye, M., Park, C., and Hazelbauer, G.L. 1994. Transmembrane signalling by a hybrid protein: communication from the domain of chemoreceptor Trg that recognizes sugar-binding proteins to the kinase/phosphatase domain of the osmosensor EnvZ. J. Bacteriol. 176, 1157-1163. This part can activate genes attached to an OmpR-responsive promoter such as PompC. To avoid inappropriate activation via EnvZ, it may be necessary to use a chassis in which EnvZ is not present. | + | Trz hybrid signal transduction protein: this was created by fusing the extracellular receptor domain of the chemoreceptor Trg with the intracellular signal transduction domain of 2-component sensor protein EnvZ, which normally phosphorylates response regulator OmpR (see Baumgartner, J.W., Kim, C., Brissette, R.E., Inouye, M., Park, C., and Hazelbauer, G.L. 1994. Transmembrane signalling by a hybrid protein: communication from the domain of chemoreceptor Trg that recognizes sugar-binding proteins to the kinase/phosphatase domain of the osmosensor EnvZ. J. Bacteriol. 176, 1157-1163). This part can activate genes attached to an OmpR-responsive promoter such as PompC. To avoid inappropriate activation via EnvZ, it may be necessary to use a chassis in which EnvZ is not present. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
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+ | Trz is a fusion protein with the extracellular region of the chemoreceptor Trg fused to the intracellular kinase domain of two-component sensor EnvZ, an osmosensor which phosphorylates the cognate response regulator OmpR. Phosphorylated OmpR then activates promoters such as the ''ompC'' promoter (available as BBa_R0082). Trg normally detects chemoattractants such as ribose, bound to the periplasmic ribose-binding protein. The usefulness of Trz arises in that ribose binding protein can be computationally redesigned to bind non-natural ligands, and this binding can then be detected via actiavtion of a reporter gene fused to the ''ompC'' promoter (Looger et al, 2003). Thus this is potentially a generalisable transduction system for detection of extracellular ligands. | ||
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+ | Trz was previously deposited as part (...) by (...); however, Registry sequence analysis of this part indicates that the sequence is not correct. It appears in fact to be ... This part is therefore a replacement for ... We have confirmed the sequence of our part but at the time of writing have not yet confirmed its activity. | ||
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Revision as of 13:13, 19 October 2009
Trz hybrid signal transduction protein
Trz hybrid signal transduction protein: this was created by fusing the extracellular receptor domain of the chemoreceptor Trg with the intracellular signal transduction domain of 2-component sensor protein EnvZ, which normally phosphorylates response regulator OmpR (see Baumgartner, J.W., Kim, C., Brissette, R.E., Inouye, M., Park, C., and Hazelbauer, G.L. 1994. Transmembrane signalling by a hybrid protein: communication from the domain of chemoreceptor Trg that recognizes sugar-binding proteins to the kinase/phosphatase domain of the osmosensor EnvZ. J. Bacteriol. 176, 1157-1163). This part can activate genes attached to an OmpR-responsive promoter such as PompC. To avoid inappropriate activation via EnvZ, it may be necessary to use a chassis in which EnvZ is not present.
Usage and Biology
Trz is a fusion protein with the extracellular region of the chemoreceptor Trg fused to the intracellular kinase domain of two-component sensor EnvZ, an osmosensor which phosphorylates the cognate response regulator OmpR. Phosphorylated OmpR then activates promoters such as the ompC promoter (available as BBa_R0082). Trg normally detects chemoattractants such as ribose, bound to the periplasmic ribose-binding protein. The usefulness of Trz arises in that ribose binding protein can be computationally redesigned to bind non-natural ligands, and this binding can then be detected via actiavtion of a reporter gene fused to the ompC promoter (Looger et al, 2003). Thus this is potentially a generalisable transduction system for detection of extracellular ligands.
Trz was previously deposited as part (...) by (...); however, Registry sequence analysis of this part indicates that the sequence is not correct. It appears in fact to be ... This part is therefore a replacement for ... We have confirmed the sequence of our part but at the time of writing have not yet confirmed its activity.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 365
- 1000COMPATIBLE WITH RFC[1000]