Difference between revisions of "Part:BBa K233324"
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This is a plasmid with a | This is a plasmid with a | ||
| − | 1) pUC 18 derived origin of replication | + | <p>1) pUC 18 derived origin of replication </p> |
| − | 2) Gene for kanamycin Resistance | + | <p>2) Gene for kanamycin Resistance</p> |
| − | 3) M13 forward and reverse primer sites | + | <p>3) M13 forward and reverse primer sites </p> |
| − | 4) Contains biobrick prefix and suffix | + | <p>4) Contains biobrick prefix and suffix</p> |
| − | + | ||
[[Image:Pidt smart.JPG |right|400px|thumbnail|Snapshot of pIDT Smart-Kan vector containing YcdB gene ]] | [[Image:Pidt smart.JPG |right|400px|thumbnail|Snapshot of pIDT Smart-Kan vector containing YcdB gene ]] | ||
| + | <br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 13:06, 19 October 2009
pIDTSmart-KAN Vector
This is a plasmid with a
1) pUC 18 derived origin of replication
2) Gene for kanamycin Resistance
3) M13 forward and reverse primer sites
4) Contains biobrick prefix and suffix
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1925
Illegal suffix found in sequence at 2 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1925
Illegal SpeI site found at 3
Illegal PstI site found at 17
Illegal NotI site found at 10
Illegal NotI site found at 1931 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1925
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1925
Illegal suffix found in sequence at 3 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1925
Illegal XbaI site found at 1940
Illegal SpeI site found at 3
Illegal PstI site found at 17 - 1000COMPATIBLE WITH RFC[1000]