Difference between revisions of "Part:BBa K233324"
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4) Contains biobrick prefix and suffix | 4) Contains biobrick prefix and suffix | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 13:01, 19 October 2009
pIDTSmart-KAN Vector
This is a plasmid with a
1) pUC 18 derived origin of replication 2) Gene for kanamycin Resistance 3) M13 forward and reverse primer sites 4) Contains biobrick prefix and suffix
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1925
Illegal suffix found in sequence at 2 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1925
Illegal SpeI site found at 3
Illegal PstI site found at 17
Illegal NotI site found at 10
Illegal NotI site found at 1931 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1925
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1925
Illegal suffix found in sequence at 3 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1925
Illegal XbaI site found at 1940
Illegal SpeI site found at 3
Illegal PstI site found at 17 - 1000COMPATIBLE WITH RFC[1000]