Difference between revisions of "Part:BBa K211002"

Revision as of 12:52, 19 October 2009

RI7-odr10 chimeric GPCR

BBa_K211002 encodes a chimeric diacetyl-sensing GPCR which can be functionally expressed in S. cerevisiae. This chimeric receptor is composed of the TM2-TM7 ligand-binding domain of the C. elegans 7-transmembrane receptor ODR-10, flanked by the N- and C- terminals of the rat OR RI7. It can be localized to the S. cerevisiae cell membrane and activate the yeast endogenous MAPK pathway upon binding to the ligand diacetyl.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 761
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K211002 short</partinfo>
-The chimeric GPCR consists of the N- and C- terminals of the rat OR RI7, flanking the TM2-TM7 ligand-binding domain of the c. elegans OR odr-10. It can be functionally expressed in S. cerevisiae, activating the yeast endogenous MAPK pathway upon binding to the ligand diacetyl.
+[https://parts.igem.org/Part:BBa_K211002 BBa_K211002] encodes a chimeric diacetyl-sensing GPCR which can be functionally expressed in ''S. cerevisiae''. This chimeric receptor is composed of the TM2-TM7 ligand-binding domain of the ''C. elegans'' 7-transmembrane receptor ODR-10, flanked by the N- and C- terminals of the rat OR RI7. It can be localized to the ''S. cerevisiae'' cell membrane and activate the yeast endogenous MAPK pathway upon binding to the ligand diacetyl.
+[[Image:Chimera.png]]
 <!-- Add more about the biology of this part here