Difference between revisions of "Part:BBa K233324"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K233324 short</partinfo>
 
<partinfo>BBa_K233324 short</partinfo>
Line 7: Line 6:
 
1) pUC 18 derived origin of replication  
 
1) pUC 18 derived origin of replication  
 
2) Gene for kanamycin Resistance
 
2) Gene for kanamycin Resistance
−
3) M13 forward and reverse primer sites (Forward primer 7-22; Reverse Primer 369-386 nu)
+
3) M13 forward and reverse primer sites  
−
 
+
4) Contains biobrick prefix and suffix
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 12:42, 19 October 2009

pIDTSmart-KAN Vector

This is a plasmid with a

1) pUC 18 derived origin of replication 2) Gene for kanamycin Resistance 3) M13 forward and reverse primer sites 4) Contains biobrick prefix and suffix

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1925
    Illegal suffix found in sequence at 2
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1925
    Illegal SpeI site found at 3
    Illegal PstI site found at 17
    Illegal NotI site found at 10
    Illegal NotI site found at 1931
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1925
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1925
    Illegal suffix found in sequence at 3
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1925
    Illegal XbaI site found at 1940
    Illegal SpeI site found at 3
    Illegal PstI site found at 17
  • 1000
    COMPATIBLE WITH RFC[1000]